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Bergamo II microscopes accept infinity-corrected objectives with M34 x 1.0, M32 x 0.75, M25 x 0.75, or RMS threads. Together, these options encompass the majority of low-magnification, high-NA objectives used in multiphoton microscopy. With a large field number of 20, our scan optics completely utilize the optical designs of these specialized.


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Explore the features and benefits of Thorlabs Bergamo II Series Multiphoton Microscopes, designed for in vivo imaging of biological samples. Download the brochure to learn more about the laser scanning, widefield imaging, and transmitted light imaging capabilities, as well as the scan paths, scan speeds, and optical configurations of these versatile systems.


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Bergamo II multiphoton imaging platform: a completely modular system that adapts to a wide range of experimental setups. 1.2 mm In Vivo Deep Brain Volume a Establishing a Patch in a Neuronb Image Credits Cover Page Top Left: Two-photon image of neurons expressing thy1-YFP in a cleared region of the hippocampus. Courtesy of the 2017 Imaging.


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Thorlabs Bergamo II Multi-photon Microscope and Discovery Laser. The creation of transgenic animals expressing multiple fluorophores, including red-shifted fluorophores, is an important development in the field of in vivo imaging. In order to take advantage of these advances, researchers need access to two-photon lasers that supply adequate.


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The mesoscope body allows -20° to +20° rotation for the objective, as well as 2" of fine X motion, 6" of fine Y motion, and 2" of fine Z motion; just as with Thorlabs' Bergamo ® II multiphoton microscope, X, Y, and Z rotate along with the objective. A multi-jointed periscope maintains the laser alignment over the entire range of motion.


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The system was designed and built in collaboration with Thorlabs and is now a commercially available microscope system (Bergamo® II series). Then, 0.2-µm-diameter yellow-green fluorescent beads.


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Bergamo ® II Series Multiphoton Microscopy Platform Following the principle that the microscope should conform to the specimen, rather than the other way around, we created a completely modular multiphoton imaging platform that adapts to a wide range of experimental requirements.


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This unidirectional device is designed for a collimated, 1 to 2 mm (1/e 2) diameter input beam with up to 10 W of optical power and up to 500 nJ of pulse energy, enabling use with popular 1 µm high-pulse-energy photoactivation lasers. These modulators are available with either standard (>250:1) or high (>2500:1) contrast ratios for wavelength.